infinity cholesterol liquid stable reagent kit  (Thermo Fisher)

Journal: bioRxiv

Article Title: Dietary depletion of glutamine is atheroprotective

doi: 10.64898/2026.03.06.710174

Figure Lengend Snippet: ( A ) Experimental design. Apoe -/- male mice were fed WD and received drinking water with no additives (Ctrl) or water supplemented with glutamine (Gln), or alpha-ketoglutarate (AKG) for 8 weeks. ( B ) Oil red O analysis of plaque burden in the aorta with ( C ) representative images of aortas from male mice. Scale bar: 2 mm. ( D ) Plaque area at 7 locations across the aortic root from the first appearance of the aortic valves in (top) male and (bottom) female mice. ( E ) Representative images of aortic root plaques from male and female mice in control (Ctrl) and glutamine (Gln) group. Scale bar: 200 μm. Black dashed line marks representative plaques boundaries. ( F ) Total plaque volume in the aortic root, calculated as the area under the curve from (D). ( G ) Sirius red analysis of plaque collagen content. ( H ) Necrotic core size analysis at the point of peak stenosis. ( I ) Plasma cholesterol. ( J ) Glutamine plasma concentration normalized to sex matched controls. (B, F, G, I, J) Data analyzed using one-way ANOVA with Dunnett’s correction or (H) Kruskal Wallis with Dunn’s correction for post-hoc analysis with N ≥ 13. Error bars represent mean ± SEM or (H) median ± IQR. *** P < 0.001; ** P = 0.001 to 0.01; * P = 0.01 to 0.05; ns, not significant.

Article Snippet: Plasma cholesterol was analyzed, using Infinity Cholesterol Liquid Stable Reagent kit (Thermo Scientific; cat#: TR13421) and Data-Trol A, Abnormal Control Serum (Thermo Scientific; cat#: TR41001), as an internal standard, according to the manufacturer’s instructions.

Techniques: Control, Clinical Proteomics, Concentration Assay

Journal: bioRxiv

Article Title: Gene dosage imbalance disrupts systemic metabolism in the Dp16 Down syndrome mouse model

doi: 10.64898/2026.01.13.699318

Figure Lengend Snippet: A-B) Overnight fasting insulin, blood glucose, serum triglyceride, cholesterol, non-esterified free fatty acids (NEFA), and β-hydroxybutyrate (ketone) in male (A) and female (B) Dp16 and WT mice. Sample size for male mice (WT = 15; Dp16 = 12) and female mice (WT = 14; Dp16 = 15). C-F) Impaired glucose tolerance as determined by the glucose tolerance test (GTT) in male (C) and female (E) Dp16 mice compared to WT controls. Impaired insulin sensitivity as determined by the insulin tolerance test (ITT) in male (D) and female (F) Dp16 compared to WT controls. Sample size for male mice (WT = 15; Dp16 = 12) and female mice (WT = 14; Dp16 = 15). G-H) Impaired triglyceride clearance in response to lipid gavage as determined by the lipid tolerance test (LTT) in male (G) and female (H) Dp16 relative to WT controls. Sample size for male mice (WT = 10; Dp16 = 14) and female mice (WT = 15; Dp16 = 15). I-J) Pooled mouse sera from male (I) and female (J) Dp16 and WT mice were fractionated by fast protein liquid chromatography (FPLC), and the triglyceride and cholesterol content of each fraction was quantified. Fractions corresponding to very-low density lipoprotein (VLDL), low-density lipoprotein (LDL), intermediate-density lipoprotein (IDL), and high-density lipoprotein (HDL) are indicated. All data are presented as mean ± SEM. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001. For all tolerance tests, data were analyzed by 2-way ANOVA with Sidek post hoc tests.

Article Snippet: Following tissue extraction, total cholesterol content was quantified using the Infinity Cholesterol Reagent kit (Thermo Fisher Scientific, Middletown, VA) according to the manufacturer’s instructions.

Techniques: Fast Protein Liquid Chromatography

Journal: bioRxiv

Article Title: Gene dosage imbalance disrupts systemic metabolism in the Dp16 Down syndrome mouse model

doi: 10.64898/2026.01.13.699318

Figure Lengend Snippet: Quantification of hepatic TAG and DAG (by TLC method), and cholesterol (by infinity assay kit) levels in chow-fed Dp16 male (A-C) and female mice (D-F) and their corresponding WT controls. Sample size: male WT = 10 and Dp16 = 30; female WT = 15 and Dp16 = 10.

Article Snippet: Following tissue extraction, total cholesterol content was quantified using the Infinity Cholesterol Reagent kit (Thermo Fisher Scientific, Middletown, VA) according to the manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: Gene dosage imbalance disrupts systemic metabolism in the Dp16 Down syndrome mouse model

doi: 10.64898/2026.01.13.699318

Figure Lengend Snippet: A-B) Overnight fasting insulin, blood glucose, serum triglyceride, cholesterol, non-esterified free fatty acids (NEFA), and β-hydroxybutyrate (ketone) in male (A) and female (B) Dp16 and WT mice on HFD. Sample size for male mice (WT = 15; Dp16 = 12) and female mice (WT = 14; Dp16 = 14). C-F) Exacerbated glucose intolerance as determined by the glucose tolerance test (GTT) in male (C) and female (E) Dp16 compared to WT controls on HFD. Exacerbated insulin resistance as determined by the insulin tolerance test (ITT) in male (D) and female (F) Dp16 compared to WT controls. Sample size for male mice (WT = 15; Dp16 = 12) and female mice (WT = 14; Dp16 = 14). G-H) The rate of triglyceride clearance in response to lipid gavage as determined by the lipid tolerance test (LTT) in male (G) and female (H) Dp16 and WT mice. Sample size for male mice (WT = 15; Dp16 = 12) and female mice (WT = 14; Dp16 = 14). I-J) Pooled mouse sera from male (I) and female (J) Dp16 and WT mice were fractionated by fast protein liquid chromatography (FPLC), and the triglyceride and cholesterol content of each fraction was quantified. Fractions corresponding to very-low density lipoprotein (VLDL), low-density lipoprotein (LDL), intermediate-density lipoprotein (IDL), and high-density lipoprotein (HDL) are indicated. All data are presented as mean ± SEM. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001. For all tolerance tests, data were analyzed by 2-way ANOVA with Sidek post hoc tests.

Article Snippet: Following tissue extraction, total cholesterol content was quantified using the Infinity Cholesterol Reagent kit (Thermo Fisher Scientific, Middletown, VA) according to the manufacturer’s instructions.

Techniques: Fast Protein Liquid Chromatography

Journal: Hepatology (Baltimore, Md.)

Article Title: Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice

doi: 10.1002/hep.30765

Figure Lengend Snippet: Metabolic parameters in control and LivDgat2KO mice on chow and FPC diets. (A) LivDgat2KO and control mice have similar body weights on chow or FPC diet for 16 weeks. Liver to body weight is lower in LivDgat2KO animals fed the FPC diet. Age 24 weeks, n = 7–10/genotype on chow diet, n = 10 females/genotype on FPC, 15–18 males/genotype on FPC. (B) Glucose and insulin tolerance tests performed in mice fed an FPC diet for 14–15 weeks. For GTT, mice were fasted overnight, then 2 mg/g of glucose was administered intraperitoneally, and blood glucose measured at times as indicated. For ITT, mice were fasted for 4 hours, then 1 U/kg of insulin was administered intraperitoneally, and blood glucose measured at times as indicated. n = 9–13 males/genotype, n = 8–15 females/genotype. (C) Plasma TG, cholesterol (Chol), and HDL-C levels in mice fed chow or FPC diets for 16 weeks. n = 5 males/genotype, n = 4–5 females/genotype on chow diet. n = 11–16 males/genotype, n = 7 females/genotype on FPC diet. (D) Plasma TG levels. After a 4-hour fast, mice were administered Tyloxapol at time 0 and subsequent TG levels were measured at indicated time points. n = 6 males/genotype, n = 5 females/genotype. (E) Real-time PCR analysis of apolipoprotein genes in mice on chow and FPC diets. Apoa1 gene expression is decreased in LivDgat2KO animals on FPC diet. n = 4–5 females and males/genotype on chow, n = 10 females/genotype, 15–18 males/genotype on FPC. (F) Immunoblotting showing less apo-A1 in plasma of male mice on an FPC diet. Ponceau stain, with albumin band, of the same blotting is also shown. n = 4/genotype. *Indicates statistical difference between diets, *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001. +Indicates statistical difference between genotypes, +P < 0.05; ++P < 0.005; +++P < 0.0005; ++++P < 0.0001. Abbreviations: GTT, glucose tolerance test; ITT, insulin tolerance test.

Article Snippet: TG and cholesterol were measured using Infinity Triglycerides Liquid Stable Reagent and Infinity Cholesterol Liquid Stable Reagent kits (Thermo Fisher Scientific).

Techniques: Control, Clinical Proteomics, Real-time Polymerase Chain Reaction, Gene Expression, Western Blot, Staining

Journal: Hepatology (Baltimore, Md.)

Article Title: Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice

doi: 10.1002/hep.30765

Figure Lengend Snippet: LivDgat2KO mice have improved steatosis on FPC diet. (A) Representative ORO-stained liver sections and steatosis severity scores in mice on FPC diet for 16 weeks. Each symbol represents the score for an individual mouse. n = 10 females/genotype, 15–18 males/genotype. (B) Hepatic TG and total cholesterol content in mice on FPC diet for 16 weeks. n = 8/genotype/sex. (C) Real-time PCR analysis of TG synthesis genes, Dgat1 and Dgat2 in this cohort. (D) De novo lipogenesis gene expression measured by qPCR and relative protein amounts detected by mass spectrometry in mice on chow and FPC diets. (E) Beta-oxidation gene expression measured by qPCR and relative protein amounts detected by mass spectrometry in mice on chow and FPC diets. n = 4–5 females and males/genotype on chow. n = 10 females/genotype, 15–18 males/genotype on FPC for gene expression data. n = 3 males/genotype/diet for proteomic data. *Indicates statistical difference between diets, *P < 0.05; **P < 0.005; ****P < 0.0001. +Indicates statistical difference between genotypes, +P < 0.05; ++P < 0.005; +++P < 0.0005; ++++P < 0.0001. Abbreviations: Acac, acyl-CoA carboxylase; Acadl, acyl-CoA dehydrogenase long chain; Acadm, acyl-CoA dehydrogenase medium chain; Acox, acyl Co-A oxidase; CV, central vein; Chol, cholesterol; Fasn, fatty acid synthase; Ppargc1α, peroxisome proliferator-activated receptor γ co-activator 1α; PT, portal triad.

Article Snippet: TG and cholesterol were measured using Infinity Triglycerides Liquid Stable Reagent and Infinity Cholesterol Liquid Stable Reagent kits (Thermo Fisher Scientific).

Techniques: Staining, Real-time Polymerase Chain Reaction, Gene Expression, Mass Spectrometry

Journal: Hepatology (Baltimore, Md.)

Article Title: Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice

doi: 10.1002/hep.30765

Figure Lengend Snippet: Summary of lipid class changes in LivDgat2KO mice fed an FPC diet. (A) Changes in lipid class abundance and palmitate content of lipids. Data are displayed as fold change compared to chow-fed control mice (Chow Control). Bold in lower left panel indicates significant difference between Chow Control and FPC Control. Bold Italic indicates significant difference between genotypes. (B) Change in cholesterol esters between the groups, compared to chow-fed control mice. N = 4–5 male mice/group. *Indicates statistical difference between diets, ****P < 0.0001. +Indicates statistical difference between genotypes, ++++P < 0.0001. Abbreviations: G3P, glycerol 3-phosphate; LCB, long-chain sphingoid bases; Cer, ceramide; SM, sphingomyelin; LPA, lysophosphatidic acid; PA, phosphatidic acid; DAG, diacylglycerol; TAG, triglyceride; PC, phosphatidylcholine; LPC, lysophophatidylcholine; PE, phosphatidylethanolamine; LPE, lysophosphatidylethanolamine; CDP-DAG, cytidine diphosphate diacylglycerol; PI, phosphatidylinositol; LPI, lysophosphatidylinositol; PG, phosphatidylglycerol; LPG, lysophosphatidylglycerol; PS, phosphatidylserine; LPS, lysophosphatidylserine.

Article Snippet: TG and cholesterol were measured using Infinity Triglycerides Liquid Stable Reagent and Infinity Cholesterol Liquid Stable Reagent kits (Thermo Fisher Scientific).

Techniques: Control